LabBench Activity

The Spectrophotometer

The spectrophotometer is an instrument that can be adjusted to illuminate a sample with a specific wavelength of light. The spectrophotometer then measures the amount of light energy that is absorbed or transmitted by the sample. In this laboratory, as DPIP goes from blue to colorless, the amount of light of wavelength 605 nm transmitted through the sample will increase. Though you can note this color change visually, the spectrophotometer quantifies the change.

Operation of the Spectrophotometer